Microarray data normalization and transformation
Microarray experiments analyse biological systems under controlled conditions and try to infer biologically meaningful information from the differences observed between gene expression profiles. Actually, one gets spot fluorescence profiles, related but not exactly the mRNA concentration in the samples studied. This talk will focus on the relation between the spot fluorescence values and the desired mRNA concentration, the factors that affect this relation and how to deal with them during the data analysis step. Data normalization and transformation procedures will be discussed.